In acquiring all-natural dietderived TLR8 Agonist site antioxidant to prevent oxidative harm [18,19]. Thus several research have been carried out on organic sources to unravel the components which possess antioxidant properties and with low cytotoxicities . Organic antioxidants are frequently additional desirable for consumption than the synthetic a single for example butylated hydroxyanisole (BHA) which was reported to become carcinogenic to humans . Not too long ago, numerous research have been carried out on the antioxidant properties of phenolic compounds which have aroused increasing interest within the isolation of such compounds present inside the plants . Cancer is often a genetic disease, which is primarily driven by genetic instability, like modifications in oncogenes and tumor suppressor genes which leads to the expression of abnormal proteins involved within the stimulation of cell proliferation and survival [23,24]. A big body of evidences have shown that free of charge radicals happen to be implicated within the development of cancer in humans [25,26]. One particular instance of your free radicals, could be the hydroxyl radical which may cause genetic mutation by forming adduct with guanine to kind hydroxylated bases of DNA (8 hydroxyl-2-deoxyguanosine) causing transversions of GC (guanine-cytosine) to TA (thymine-adenine) [27,28]. Epidemiologic research have also shown that cancer could possibly be as a consequence of quite a few things which include exposure to environmental carcinogenic agents, way of life (tobacco and alcohol consumption), nutritional habit and infectious agents [29-32]. These things can initiate and promote carcinogenesis which may progress to cancer. To the most effective of our understanding, there is no antioxidant and cytotoxic investigation on extracts of this species. As a result, this paper reports the antioxidant and cytotoxic activities of the crude aqueous methanol and fractionated extracts in the rhizomes of Alpinia pahangensis and to determine the phenolic content. This study also aims to correlate the phenolic content material on the crude and fractionated extracts with its antioxidant properties. The active extract was further subjected to gas chromatography ass spectrometry (GC-MS) analysis for identification with the elements present within the extract.MethodsSample collectionThe rhizomes of Alpinia pahangensis have been collected from Pahang, Malaysia. This species was authenticated by Professor Dr Halijah Ibrahim, from Faculty of Science, University of Malaya and also a voucher specimen (No. KLU 46177) deposited inside the university herbarium.Phang et al. BMC Complementary and Alternative Medicine 2013, 13:243 biomedcentral/1472-6882/13/Page 3 ofReagents and chemicalsButylated hydroxyanisole (BHA), ascorbic acid, FolinCiocalteu’s phenol reagent, -carotene, linoleic acid, Tween 80, gallic acid and two,2-diphenyl-1-picrylhydrazyl (DPPH), potassium ferricyanide have been acquired from Sigma-Aldrich. Methanol, hexane, ethyl PRMT1 Inhibitor Purity & Documentation acetate and trichloroacetic acid were obtained from Merck. All solvents were bought in analytical grade.Human cell line and culture medium. The crude methanolic extract, hexane fraction, ethyl acetate fraction and positive manage (BHA and ascorbic acid) had been dissolved in methanol even though water fraction was dissolved in distilled water. The total phenolic content material (mg/ g of plant extract) inside the crude aqueous methanol extract and its fractions expressed in gallic acid equivalents (GAE). Mean values had been calculated from 3 measurement.DPPH radical scavenging assayThe cell lines have been bought in the American Tissue Culture Collection (ATCC, USA).